Il-Shin Kim has completed her PhD at the age of 28 years from Chonnam National University School of Dentistry. She is the professor of Honam University. She is interested in oral health education and prevention of periodontal disease.
Proteasome inhibitors are drugs that block the action of proteasomes which mainly functions in the break down of cellular complexes proteins, like p53 protein.This study investigated the effects of proteasome inhibitor-1 and epoxomicin on osteoblast differentiation in periodontal ligament (PDL) cells and its mechanism. Mouse PDL cells were cultured in DMEM (growth medium containing 10% fetal bovine serum and 1% antibiotics) with proteasome inhibitor-1 or epoxomicin. The cells were pre-treated with TNF-α. After 2 days of TNF-α treatment, they were treated with not only proteasome inhibitors but also BMP-2. Metabolic cell activity after 24 hr was measured using WST-1 assay. Real-time PCR were performed to analyze the expression of several genes involved in osteoblast differentiation. Bone forming ability in cell cultures was characterized by examining alkaline phosphatase (ALP) activity and ALP staining. Activation of phosphor-Smad 1/5/8 and Smad antibodies were determined by western blot analysis. After treatment of TNF-α, BMP-2 with proteasome inhibitor-1 or epoxomicin resulted in the recovery of genes like ALP and bone sialoprotein. Overall, proteasome inhibitors-treated cells increased the expression of ALP and mineralized nodule formation when compared to other controls. Activation of Smad signaling pathway was observed. In the present study, proteasome inhibitors improve the osteoblastic differentiation which was decreased by TNF-α. This suggests that proteasome inhibitors (proteasome inhibitor-1 and epoxomicin) could be a promising therapeutic approach for periodontal diseases.
Dr.Mahsa Ghorbani has completed his PhD at the age of 27 years from Shiraz University of Medical Sciences, Shiraz, Iran,and Endodontist specialty studies from Shiraz University. She has published some papers in Iranian journals.
Scientometrics is the study of metrics mainly measuring and analysing science, technology and innovation and focuses on understanding the scientific citations, mapping scientific fields and production of indicators for use in policy and management contexts. The present study was designed to collect the statistical information of endodontic treatment articles by Iranian authors published in PubMed. We searched the PubMed database for endodontic treatment articles globally from February 01, 2016 until February 01, 2018. According to MeSH keyword, Endodontic Treatment was the best keyword to do primary search. After that, the limitation with Clinical Trials and combination with Iran in affiliation field, all abstracts categorized. The data were further analyzed to illustrate the related scientometric indicators. At first, a total of 2,884 articles were retrieved according to all global authors. The proportion of Iranian authors was 10.88% (No.=265). The number of PubMed-indexed publications between 2013 and 2018 showed only 144 (4.99%) articles which were categorized as Clinical Trial. No article was retrieved by Iran author affiliation. According to phases of clinical trial research designs, only 1 article in Clinical Trial Phase I, and 1 article in Clinical Trial Phase II were indexed in subcategory of Clinical Trials, globally. With limitation to Review article, totally, 384 (13.31%) articles were found. By using limitation, the number of Review articles with Iran affiliation were 36 (9.37%). Analysis of PubMed-indexed endodontic treatment publications originating from all of the word in relation to Iranian studies revealed it is necessary a growing trend toward clinical trials studies in Iran. Although, the Iranian proportion in Review articles could be rise in future.